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| Registros recuperados: 181 | |
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| Mateos, Eduardo; Giribet, Gonzalo; Carranza, Salvador. |
| The first records of terrestrial planarians belonging to the family Rhynchodemidae are reported for the Iberian Peninsula. A new endemic species from the Spanish Pyrenees, Microplana nana sp. nov., is described. The characteristic features of this species are: i) small size (8-10 mm) in adult individuals, ii) very long conical penis papilla and iii) absence of seminal vesicle, bursa copulatrix, genito-intestinal duct, and well-developed penial bulb. Moreover, the widespread common European land planarian Microplana terrestris (Müller, 1774) is reported for the first time from the Iberian Peninsula. The two species, M. nana sp. nov. and M. terrestris, are described by means of external morphology using histological sections, and have been characterized by... |
| Tipo: Article / Letter to the editor |
Palavras-chave: New species; ITS-1; Molecular markers; Iberian peninsula; Microplana; Rhynchodemidae; Terricola. |
| Ano: 1998 |
URL: http://www.repository.naturalis.nl/record/534286 |
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| Della Vecchia,P.T.; Silva,C.A.R. da; Terenciano-Sobrinho,P.. |
| Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Seed testing; Seed companies; Genetic purity. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500014 |
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| Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S.. |
| The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams & Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012 |
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| Martins Filho,Sebastião; Sediyama,Carlos Sigueyuki; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de. |
| The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; RAPD; SCAR; Soybean; Cercospora sojina; Frogeye leaf spot. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012 |
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| Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G.. |
| Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010 |
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| Santos,Carlos A.F.; Simon,Philipp W.. |
| Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Daucus carota; Evolution; Molecular markers; SCAR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013 |
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| Garcia Meunier, Pascale; Martel, Corine; Pigeot, Jacques; Chevalier, Guillemette; Blanchard, Gérard; Goulletquer, Philippe; Robert, Stephane; Sauriau, Pierre-guy. |
| The direct amplification of length polymorphism technique (DALP) has been used to distinguish species-specific banding patterns in two marine gastropod oyster drills Ocenebra erinacea (Linnaeus, 1758) and Ocinebrellus inornatus (Recluz, 1851). Ocenebra erinacea is the European oyster drill, common along all European coasts. Ocinebrellus inornatus, the Japanese oyster drill, was recorded in oyster growing areas of the Marennes-Oleron Bay (SW France) for the first time in 1995. This new biological invasion could lead to an increase, which must be evaluated, in the predation risk for cultivated species i.e. oysters and blue mussels, and for littoral fishing resources along the French Atlantic coasts. As a result, since specific identification of early life... |
| Tipo: Text |
Palavras-chave: French Atlantic coast; Ocenebra erinacea; Ocinebrellus inornatus; Marine bioinvasions; Molecular markers. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-569.pdf |
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| Menezes,Carlos C.E; Vantoai,Tara; McDonald,Miller B; Sediyama,Tocio. |
| Sequence-Characterized Amplified Region (SCAR) appears as a useful technique for genetic purity testing and variety discrimination, applicable to species in which some other techniques have failed. In particular, this technique is very attractive with species in which RAPD results were not consistent. The RAPD polymorphic bands were cloned, sequenced and from the sequence information, primers pairs for normal PCR were developed. Since the probability of obtaining successful SCAR primers from RAPD polymorphic bands was about 50%, a larger number of RAPD polymorphic bands are needed to develop sufficient SCAR primers for varietal discrimination in vinca. In addition, the efficiency of the SCAR technique is strongly affected by the quality of DNA extracted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vinca; Seed; Variety discrimination; Molecular markers; SCAR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100041 |
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| Clement,Charles R.; Sousa,Nelcimar Reis; Rodrigues,Doriane Picanço; Astolfi-Filho,Spártaco; Moreno,Yolanda Núñez; Pascual,Vicente Torres; Rodríguez,Francisco Javier Gallego. |
| Although the first inhabitants of western Amazonia domesticated pejibaye (Bactris gasipaes Kunth, Palmae) or peach palm for its fruits, today it is widely planted for its heart-of-palm. Like other domesticates, pejibaye presents a complex hierarchy of landraces developed before the conquest of the Americas. The existence of three landraces (Pará, Solimões, Putumayo) was proposed along the Amazonas and Solimões Rivers, Brazil, based on morphological characteristics. There are some questions remaining about the intermediate landrace being an artifact of the morphometric analysis. AFLPs were used to evaluate the relationships among samples of these putative landraces. DNA was extracted from 99 plants representing 13 populations maintained in the Pejibaye... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Population differentiation; Genetic resources; Genetic analysis. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000400019 |
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| Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de. |
| A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Population genetics; Plant breeding; Molecular markers. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022 |
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| Registros recuperados: 181 | |
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